Maedi-visna virus Vif protein uses motifs distinct from HIV-1 Vif to bind zinc and cofactor required for A3 degradation

The mammalian apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3 or A3) household of cytidine deaminases limit viral infections by mutating viral DNA and impeding reverse transcription. To beat this antiviral exercise, most lentiviruses categorical a viral accent protein referred to as Vif, which recruits A3 proteins to Cullin-RING E3 ubiquitin ligases equivalent to Cul5 for ubiquitylation and subsequent proteasomal degradation.

Whereas Vif proteins from primate lentiviruses like HIV-1 make the most of the transcription issue CBFβ as a non-canonical cofactor to stabilize the advanced, maedi-visna virus (MVV) Vif hijacks cyclophilin A (CypA) as an alternative. Since CBFβ and CypA are each extremely conserved amongst mammals, the requirement for 2 completely different mobile cofactors means that these two A3-targeting Vif proteins have completely different biochemical and structural properties.

To research this matter, we used a mix of in vitro biochemical assays and in vivo A3 degradation assays to review motifs required for MVV Vif to bind zinc ion, Cul5, and the cofactor CypA. Our outcomes exhibit that whereas some widespread motifs between HIV-1 Vif and MVV Vif are concerned in recruiting Cul5, completely different determinants in MVV Vif are required for cofactor binding and stabilization of the E3 ligase advanced, such because the zinc-binding motif and N- and C-terminal areas of the protein.

Outcomes from this research advance our understanding of the mechanism of MVV Vif recruitment of mobile components and the evolution of lentiviral Vif proteins. Widespread or European hedgehogs could be discovered all through Western Europe. They’re identified carriers of a wide range of parasitic and bacterial pathogens, and have additionally been proven to hold a number of viruses, together with morbilli-like paramyxoviruses, though the pathogenic and zoonotic potential of a few of these viruses has but to be decided. We report right here the invention of a novel paramyxovirus in Belgian hedgehogs, named Belerina virus.

The virus was detected by nanopore sequencing of RNA remoted from hedgehog tissue. Out of 147 animals screened on this research, 57 examined optimistic for Belerina virus (39%), indicating a excessive prevalence of this virus within the Belgian hedgehog inhabitants. Primarily based on its divergence from different identified paramyxovirus species, Belerina virus is believed to characterize a brand new species within the household Paramyxoviridae.

 

[Co-expression, purification and bioassay of three avian viral antigens]

To attain uniform soluble expression of a number of proteins in the identical Escherichia coli pressure, and simplify the method steps of antigen manufacturing in genetic engineering subunit multivalent vaccine, we co-expressed three avian virus proteins together with the fowl adenovirus serotype 4 (FAdV-4) Fiber-2 protein, infectious bursal illness virus (IBDV) VP2 protein and egg-drop syndrome virus (EDSV) Fiber protein in E. coli BL21(DE3) cells after optimization of gene codon, promoter, and tandem expression order. The purified proteins had been analyzed by Western blotting and agar gel precipitation (AGP).
The content material of the three proteins had been well-proportioned after co-expression and the purity of the purified proteins had been greater than 80%. Western blotting evaluation and AGP experiment outcomes present that each one the three co-expression proteins had immunoreactivity and antigenicity.
It’s the first time to realize the three completely different avian virus antigens co-expression and co-purification, which simplified the method of antigen manufacturing and laid a basis for the event of genetic engineering subunit multivalent vaccine.

Maedi-visna virus Vif protein uses motifs distinct from HIV-1 Vif to bind zinc and cofactor required for A3 degradation
Maedi-visna virus Vif protein uses motifs distinct from HIV-1 Vif to bind zinc and cofactor required for A3 degradation

[Generation and evaluation of a recombinant myxomavirus expressing the VP60 protein of rabbit haemorrhagic disease virus]

Rabbit haemorrhagic illness virus (RHDV) and myxoma virus (MYXV), are two pathogens which have dangerous impact on rabbit breeding and inhabitants decline of European rabbits of their native vary, inflicting rabbit haemorrhagic illness (rabbit fever) and myxomatosis, respectively.
The capsid protein VP60 of the RHDV represents the most important antigenic protein. To develop a recombinant bivalent vaccine candidate that may concurrently forestall these two illnesses, we used the nonessential gene TK (thymidine kinase) of MYXV because the insertion web site to assemble a recombinant shuttle vector p7.5-VP60-GFP expressing the RHDV main capsid protein (VP60) and the selectable marker GFP.
Then the shuttle vector p7.5-VP60-GFP was transfected into rabbit kidney cell line RK13 which was beforehand contaminated with MYXV. After homologous recombination, the recombinant virus expressing GFP was screened beneath a fluorescence microscope and named as rMV-VP60-GFP. Lastly, the precise gene-knock in and expression verification of the vp60 and gfp genes of the recombinant virus was confirmed by PCR and Western blotting.

LIMITED QTY-Dinucleotide Standards Formamido Thymine Dimer d (PfpT) (50 µg)

0801212 50 µg
EUR 118.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Formamido Adenine Dimer d (PfpA) (50 µg)

0801213 50 µg
EUR 118.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Thymine Glycol Cytidine Dimer d (TgpC) (50µg)

0801216 50µg
EUR 118.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Thymine Glycol Guanine Dimer d (TgpG) (50 µg)

0801214 50 µg
EUR 118.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Thymine Glycol Thymine Dimer d (TgpT) (50 µg)

0801215 50 µg
EUR 118.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Thymine Glycol Adenine Dimer d (TgpA) (50 µg)

0801217 50 µg
EUR 118.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-rhIL-2 Recombinant Human Interleukin-2 (100 µg)

0802001 100 µg
EUR 362
Description: Please contact Gentaur in order to receive the datasheet of the product.

Dopamine (DA) ELISA Kit

DLR-DA-Ge-48T 48T
EUR 568
Description: A competitive inhibition quantitative ELISA assay kit for detection of Dopamine (DA) in samples from serum, plasma, tissue homogenates or other biological fluids.

Dopamine (DA) ELISA Kit

DLR-DA-Ge-96T 96T
EUR 744
Description: A competitive inhibition quantitative ELISA assay kit for detection of Dopamine (DA) in samples from serum, plasma, tissue homogenates or other biological fluids.

General Dopamine (DA) ELISA Kit

RD-DA-Ge-48Tests 48 Tests
EUR 579

General Dopamine (DA) ELISA Kit

RD-DA-Ge-96Tests 96 Tests
EUR 806

General Dopamine (DA) ELISA Kit

RDR-DA-Ge-48Tests 48 Tests
EUR 605

General Dopamine (DA) ELISA Kit

RDR-DA-Ge-96Tests 96 Tests
EUR 843

8-Oxo-GTP

B8045-.01 10 ?l (100 mM)
EUR 105

8-Oxo-GTP

B8045-.05 50 ?l (100 mM)
EUR 313

8-Oxo-GTP

B8045-.1 100 ?l (100 mM)
EUR 479

Bovine 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080b 96 Tests
EUR 928

Chicken 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080c 96 Tests
EUR 928

Canine 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080d 96 Tests
EUR 928

General 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080Ge 96 Tests
EUR 886

Human 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080h 96 Tests
EUR 824

Mouse 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080m 96 Tests
EUR 865

Porcine 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080p 96 Tests
EUR 928

Rat 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080r 96 Tests
EUR 886

Rabbit 8- Oxo- 2'- deoxyguanosine, 8- oxo- Dg ELISA Kit

CELI-66080Rb 96 Tests
EUR 928

1730 8 SNAP-SEAL 8 OZ

1730-8 100/pk
EUR 74
Description: Disposable Plastic; Plastic Containers

Individual Reaction Mix 8

G065-8 200 reactions
EUR 167

8-Oxo-DGTP Diphosphatase (mutT) Antibody

20-abx300694
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human IL-8 Recombinant Protein

R00423-8 5ug/vial
EUR 259
Description: Interleukin-8 (IL-8), also known as CXCL8, is an ELR-positive CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. Human IL-8 Recombinant Protein is purified interleukin-8 produced in yeast.

EXOCET Standards

EXOCET-SD-1
EUR 202

Escherichia coli 8-oxo-dGTP diphosphatase (mutT)

1-CSB-YP357355ENV
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli 8-oxo-dGTP diphosphatase(mutT) expressed in Yeast

Escherichia coli 8-oxo-dGTP diphosphatase (mutT)

1-CSB-EP357355ENV
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli 8-oxo-dGTP diphosphatase(mutT) expressed in E.coli

8-Oxo-dGTP Diphosphatase (MUTT) Antibody (HRP)

20-abx300695
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

8-Oxo-dGTP Diphosphatase (MUTT) Antibody (FITC)

20-abx300696
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

8-Oxo-dGTP Diphosphatase (MUTT) Antibody (Biotin)

20-abx300697
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 303.5

General 8OHDG/ 8-Oxo-2'-deoxyguanosine ELISA Kit

E0081Ge 1 Kit
EUR 717

ELISA kit for General 8-Oxo-2'-deoxyguanosine

EK2088 96 tests
EUR 603
Description: Enzyme-linked immunosorbent assay kit for quantification of General 8-Oxo-2'-deoxyguanosine in samples from serum, plasma, tissue homogenates and other biological fluids.

General 8- Oxo- 2'- deoxyguanosine, 8OHDG ELISA KIT

ELI-02314Ge 96 Tests
EUR 886

Nucleoside Phosphorylase (Nucleoside phosphorylase) Antibody

abx235893-100ug 100 ug
EUR 481

8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

DLR-8-OHdG-Ge-48T 48T
EUR 469
Description: A competitive inhibition quantitative ELISA assay kit for detection of 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma or other biological fluids.

8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

DLR-8-OHdG-Ge-96T 96T
EUR 608
Description: A competitive inhibition quantitative ELISA assay kit for detection of 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma or other biological fluids.

0.5-10UL 8-CHANNEL, DIGITAL PIPETTOR

AP-8-10 1/pk
EUR 425
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

20-200UL 8-CHANNEL, DIGITAL PIPETTOR

AP-8-200 1/pk
EUR 425
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

50-300UL 8-CHANNEL, DIGITAL PIPETTOR

AP-8-300 1/pk
EUR 425
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

5-50UL 8-CHANNEL, DIGITAL PIPETTOR

AP-8-50 1/pk
EUR 425
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RD-8-OHdG-Ge-48Tests 48 Tests
EUR 467

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RD-8-OHdG-Ge-96Tests 96 Tests
EUR 646

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RDR-8-OHdG-Ge-48Tests 48 Tests
EUR 488

General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

RDR-8-OHdG-Ge-96Tests 96 Tests
EUR 676

pAAV-DJ/8 Vector

VPK-420-DJ-8 10 µg
EUR 647
Description: The pAAV-DJ/8 vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ/8. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ/8 packaging.

3-oxo-8-tbutylcarbonyldicyclo-2-decane-7-boronic ester

abx181986-1g 1 g
EUR 2207

MRC5 Standards A-F

F373 1 ml
EUR 488
Description: MRC5 Standards A-F by Cygnus Technologies is available in Europe via Gentaur.

Da-3 Antibody

1-CSB-PA22309A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Da-3. Recognizes Da-3 from Trifolium repens. This antibody is Unconjugated. Tested in the following application: ELISA

DAF-FM DA

HY-D0717 1mg
EUR 693

Da-3 Antibody

20-abx339020
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Dopamine (DA) Antibody

20-abx100084
  • EUR 398.00
  • EUR 133.00
  • EUR 1080.00
  • EUR 537.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

tert-Butyl 1-oxo-2,8-diazaspiro[4.5]decane-8-carboxylate

20-abx180883
  • EUR 467.00
  • EUR 634.00
  • 250 mg
  • 500 mg

PSA (Prostate-specific antigen) ELISA test

8 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PSA (Prostate-specific antigen)

10-Oxo Docetaxel

HY-16674 10mg
EUR 2307

Dimethyl(oxo)phosphanium

TBW01428 unit Ask for price

BSA Standards Set, A-E

F031 1 ml
EUR 403
Description: BSA Standards Set, A-E by Cygnus Technologies is available in Europe via Gentaur.

Insulin Standards Set, A-G

F043 1 ml
EUR 488
Description: Insulin Standards Set, A-G by Cygnus Technologies is available in Europe via Gentaur.

HSA Standards Set, A-E

F057 1 ml
EUR 411
Description: HSA Standards Set, A-E by Cygnus Technologies is available in Europe via Gentaur.

S.cerevisiae Standards Set, A-F

F138 1 ml
EUR 411
Description: S.cerevisiae Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Human IgM Standards A-F

F171 1 ml
EUR 488
Description: Human IgM Standards A-F by Cygnus Technologies is available in Europe via Gentaur.

A549 Standards Set, A-F

F233 1 ml
EUR 450
Description: A549 Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Protein A Standards A-H

F743 1 ml
EUR 411
Description: Protein A Standards A-H by Cygnus Technologies is available in Europe via Gentaur.

E.coli DNA Standards A-H

D413 1 ml
EUR 322
Description: E.coli DNA Standards A-H by Cygnus Technologies is available in Europe via Gentaur.

CHO DNA Standards (A-H)

D553 1 ml
EUR 322
Description: CHO DNA Standards (A-H) by Cygnus Technologies is available in Europe via Gentaur.

BSA Conjugated Dopamine (DA)

4-CPA851Ge11
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Dopamine expressed in: Protein conjugation

OVA Conjugated Dopamine (DA)

4-CPA851Ge21
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Dopamine expressed in: Protein conjugation

Anserine DA ELISA Kit

EAD0247 96Tests
EUR 521

Rat DA ELISA Kit

ERD0247 96Tests
EUR 521

Rabbit DA ELISA Kit

ERTD0247 96Tests
EUR 521

Mouse DA ELISA Kit

EMD0247 96Tests
EUR 521

Porcine DA ELISA Kit

EPD0247 96Tests
EUR 521

Human DA ELISA Kit

EHD0247 96Tests
EUR 521

Canine DA ELISA Kit

ECD0247 96Tests
EUR 521

Goat DA ELISA Kit

EGTD0247 96Tests
EUR 521

Bovine DA ELISA Kit

EBD0247 96Tests
EUR 521

DA-PEG-Phenetidine,2K

33-HE047110-2K
  • EUR 781.00
  • EUR 21975.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Dopamine (DA) CLIA Kit

20-abx190012
  • EUR 7645.00
  • EUR 4074.00
  • EUR 942.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Dopamine (DA) ELISA Kit

abx574183-96tests 96 tests
EUR 715

Da-3 Antibody (HRP)

20-abx339021
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Da-3 Antibody (FITC)

20-abx339022
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Da-3 Antibody (Biotin)

20-abx339023
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Dopamine (DA) ELISA Kit

abx051435-96tests 96 tests
EUR 668

Dopamine (DA) ELISA Kit

20-abx150336
  • EUR 7504.00
  • EUR 3996.00
  • EUR 926.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Da-3 Polyclonal Antibody

A68767 100 ?g
EUR 628.55
Description: kits suitable for this type of research

Dopamine (DA) CLIA Kit

abx196129-96tests 96 tests
EUR 825

Dopamine (DA) Antibody (Biotin)

20-abx271740
  • EUR 411.00
  • EUR 230.00
  • EUR 1163.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Dopamine (DA) ELISA Kit

abx257130-96tests 96 tests
EUR 668

Dopamine (DA) Antibody (FITC)

20-abx273557
  • EUR 439.00
  • EUR 244.00
  • EUR 1261.00
  • EUR 606.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

DA(Dopamine) ELISA Kit

EU0392 96T
EUR 476.25
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: General;Sensitivity: 0.938 ng/ml

Dopamine (DA) ELISA Kit

K4219-100
EUR 767

AAV-DJ/8 Helper Free Packaging System

VPK-400-DJ-8 1 kit
EUR 972
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-DJ/8 Helper Free Expression System

VPK-410-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

scAAV-DJ/8 Helper Free Expression System

VPK-430-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards (1000 assays)

31028 1kit
EUR 362
Description: Minimum order quantity: 1 unit of 1kit

Recombinant E.coli 8-oxo-dGTP diphosphatase Protein, His-SUMO, E.coli-100ug

QP7301-ec-100ug 100ug
EUR 707

Recombinant E.coli 8-oxo-dGTP diphosphatase Protein, His-SUMO, E.coli-10ug

QP7301-ec-10ug 10ug
EUR 326

Recombinant E.coli 8-oxo-dGTP diphosphatase Protein, His-SUMO, E.coli-1mg

QP7301-ec-1mg 1mg
EUR 2303

Recombinant E.coli 8-oxo-dGTP diphosphatase Protein, His-SUMO, E.coli-200ug

QP7301-ec-200ug 200ug
EUR 1115

Recombinant E.coli 8-oxo-dGTP diphosphatase Protein, His-SUMO, E.coli-500ug

QP7301-ec-500ug 500ug
EUR 1514

Recombinant E.coli 8-oxo-dGTP diphosphatase Protein, His-SUMO, E.coli-50ug

QP7301-ec-50ug 50ug
EUR 435

Cordycepin (Nucleoside Antagonist)

SIH-576-10MG 10 mg
EUR 126
Description: The substance Cordycepin is a nucleoside antagonist. It is synthetically produced and has a purity of >98%. The pure substance is white powder which is May be dissolved in DMSO (25 mg/ml).

Cordycepin (Nucleoside Antagonist)

SIH-576-50MG 50 mg
EUR 277
Description: The substance Cordycepin is a nucleoside antagonist. It is synthetically produced and has a purity of >98%. The pure substance is white powder which is May be dissolved in DMSO (25 mg/ml).

Nucleoside-Analog-1

HY-77651 100mg
EUR 2943

Nucleoside-Analog-2

HY-77652 100mg
EUR 2943

anti-Nucleoside phosphorylase

YF-PA24262 50 ul
EUR 334
Description: Mouse polyclonal to Nucleoside phosphorylase

AAV-DJ/8 Helper Free Promoterless Expression System

VPK-411-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

RD-8-epi-PGF2a-Ge-48Tests 48 Tests
EUR 467

General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

RD-8-epi-PGF2a-Ge-96Tests 96 Tests
EUR 646

CHO HCP Standards Set, A-F

F018 1.5 ml
EUR 373
Description: CHO HCP Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Protein A Standards Set, A-E

F053 1 ml
EUR 411
Description: Protein A Standards Set, A-E by Cygnus Technologies is available in Europe via Gentaur.

Mouse IgG1 Standards Set, A-F

F077 1 ml
EUR 411
Description: Mouse IgG1 Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Mouse IgG2a Standards Set, A-F

F078 1 ml
EUR 411
Description: Mouse IgG2a Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Mouse IgG2b Standards Set, A-F

F079 1 ml
EUR 411
Description: Mouse IgG2b Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Mouse IgG Standards Set, A-E

F081 1 ml
EUR 411
Description: Mouse IgG Standards Set, A-E by Cygnus Technologies is available in Europe via Gentaur.

Mouse IgM Standards Set, A-F

F093 1 ml
EUR 411
Description: Mouse IgM Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Standards Set, A-F

F213 1 ml
EUR 411
Description: Goat IgG Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Standards Set, A-G

F223 1 ml
EUR 488
Description: NS/0 Standards Set, A-G by Cygnus Technologies is available in Europe via Gentaur.

Insulin-US Standards Set, A-F

F282 1 ml
EUR 488
Description: Insulin-US Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

Protein A Standards Set, A-G

F403 1 ml
EUR 411
Description: Protein A Standards Set, A-G by Cygnus Technologies is available in Europe via Gentaur.

E.coli HCP Standards Set, A-F

F413 1 ml
EUR 488
Description: E.coli HCP Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

P.fluorescens HCP Standards Set, A-F

F453 1 ml
EUR 450
Description: P.fluorescens HCP Standards Set, A-F by Cygnus Technologies is available in Europe via Gentaur.

BHK Cell HCP Standards A-F

F513 1 ml
EUR 527
Description: BHK Cell HCP Standards A-F by Cygnus Technologies is available in Europe via Gentaur.

SF9 HCP Standards Set, A-H

F843 1 ml
EUR 411
Description: SF9 HCP Standards Set, A-H by Cygnus Technologies is available in Europe via Gentaur.

NS/0 DNA Standards (A-H)

D223 1 ml
EUR 322
Description: NS/0 DNA Standards (A-H) by Cygnus Technologies is available in Europe via Gentaur.

Library Quantification DNA Standards (S1-S6)

20-abx098897
  • EUR 620.00
  • EUR 620.00
  • EUR 620.00
  • EUR 620.00
  • 25%, 120 ul
  • 37.5%, 120 ul
  • 50%, 120 ul
  • 62.5%, 120 ul

AXYGEN® 8-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-8-1 1/pk
EUR 59
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.0 MM THICKNESS

HGB15-8-1 1/pk
EUR 69
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-8-15 1/pk
EUR 69
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 7 CM GEL BOX, 0.75 MM THICKNESS

HGB7-8-075 1/pk
EUR 54
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 7 CM GEL BOX, 1.0 MM THICKNESS

HGB7-8-1 1/pk
EUR 54
Description: Lab Equipment; Axygen Branded EQ

AAV-DJ/8 Helper Free Bicistronic Expression System (Puro)

VPK-415-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

AAV-DJ/8 Helper Free Bicistronic Expression System (Neo)

VPK-416-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)

VPK-417-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

AAV-DJ/8 Helper Free Bicistronic Expression System (GFP)

VPK-418-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin)

VPK-419-DJ-8 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

AXYPET 0.5-10UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-10-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 0.5-10UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-10-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 20-200UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-200-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 20-200UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-200-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 50-300UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-300-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 50-300UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-300-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 5-50UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X4

AP-8-50-ALT 1/pk
EUR 456
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET 5-50UL 8-CHANNEL, DIGITAL PIPETTOR ISO17025 3X10

AP-8-50-STD 1/pk
EUR 507
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller
The outcomes confirmed that these two genes had been readily knocked into the MYXV genome and in addition efficiently expressed, indicating that the recombinant MYXV expressing the vp60 of RHDV was generated. Safety in opposition to MYXV problem confirmed that the recombinant virus induced detectable antibodies in opposition to MYXV which might make clear growth of the efficient vaccine.