Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution
Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. The reemergence of MPXV in 2017 (at Bayelsa state) after 39 years of no reported case in Nigeria, and the export of travelers’ monkeypox (MPX) from Nigeria to other parts of the world, in 2018 and 2019, respectively, have raised concern that MPXV may have emerged to occupy the ecological and immunological niche vacated by smallpox virus. This review X-rays the current state of knowledge pertaining the infection biology, epidemiology, and evolution of MPXV in Nigeria and worldwide, especially with regard to the human, cellular, and viral factors that modulate the virus transmission dynamics, infection, and its maintenance in nature.
This paper also elucidates the role of recombination, gene loss and gene gain in MPXV evolution, chronicles the role of signaling in MPXV infection, and reviews the current therapeutic options available for the treatment and prevention of MPX. Additionally, genome-wide phylogenetic analysis was undertaken, and we show that MPXV isolates from recent 2017 outbreak in Nigeria were monophyletic with the isolate exported to Israel from Nigeria but do not share the most recent common ancestor with isolates obtained from earlier outbreaks, in 1971 and 1978, respectively.
Finally, the review highlighted gaps in knowledge particularly the non-identification of a definitive reservoir host animal for MPXV and proposed future research endeavors to address the unresolved questions. Although human polyomavirus JC (JCV) seroprevalence in the general population is high, its neurological complications are rare and progressive multifocal leukoencephalopathy (PML), a lethal central nervous system (CNS) demyelinating disease, is the most well-known. After an usually asymptomatic primary infection during late childhood, a latent JCV form persists in different sites, notably the kidneys and lymphocytes.
Rearrangement of that archetype into the prototypical neurotropic strain can reactivate JCV, thereby enabling its CNS penetration and infection of glial cells. In a context of defective immune defenses (HIV infection, cancer or immunosuppressant therapies) this infection leads to oligodendrocyte death that contributes, via demyelinization, to PML but also, as more recently described, to other CNS complications, e.g., JCV granule cell neuronopathy, meningitis or encephalitis. Clinical manifestations depend on the localization of the lesions.
The increasingly widespread use of new immunomodulatory monoclonal antibodies to treat multiple sclerosis and other inflammatory systemic diseases has increased PML frequency in those previously rarely affected entities.
Can Zika Virus Infection in High Risk Pregnant Women Be Differentiated on the Basis of Symptoms?
Zika virus (ZIKV) infection in pregnancy is associated with congenital neurological abnormalities. Our understanding of the full clinical spectrum of ZIKV infection is incomplete. Using data from this prospective cohort study consisting of 650 women attending a high-risk pregnancy clinic during the Zika virus outbreak in Brazil, we investigated the extent to which specific symptoms can be utilized to differentiate ZIKV-infected pregnant women from those with other pregnancy-related problems.
All were tested for ZIKV in urine by RT-qPCR. Demographic and clinical data including physical symptoms during follow-up were recorded and analyzed with respect to Zika virus exposure status. Forty-eight (7.4%) women were positive for ZIKV by RT-qPCR. The majority (70.8%) were asymptomatic, and only four ZIKV-positive women (8.3%) reported symptoms during pregnancy that met the WHO case definition.
Zika-positive and -negative women reported similar frequencies of ZIKV-like symptoms (as per the WHO definition): fever (16.7% vs. 13.6%), arthralgia/arthritis (10.4% vs. 11.3%), rash (4.2% vs. 5.3%), and conjunctivitis (2.1% vs. 3.2%). Most pregnant women positive for ZIKV in urine are asymptomatic and do not deliver a baby with microcephaly. Physical symptoms alone did not differentiate between high-risk pregnant women positive or negative for ZIKV.
Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution
Persistent viral shedding despite seroconversion in a kidney transplant recipient with severe extrapulmonary COVID-19
Renal transplant (RT) recipients are at increased risk for infectious complications. The clinical course of COVID-19 has been described in several RT recipients with varying clinical outcomes. Most present with pulmonary manifestations, however extrapulmonary presentations are not uncommon. Also, the timing and efficacy of seroconversion in transplant recipients is not well known.
This report describes the duration of viral shedding and timing of seroconversion in a young adult RT recipient with COVID-19 who presented with severe diarrhoea and acute kidney injury requiring dialysis. She developed anti-SARS-CoV-2 IgG antibody after 5 weeks despite persistently shedding the virus in the nasopharynx until 6 weeks after symptom onset. Further studies are needed to determine if immunosuppressed patients have prolonged viral shedding and are still contagious despite seroconversion.
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.
Description: D-Dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-Dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-Dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.
Description: The CD Human D-DIMER ELISA Kit is an enzyme-linked immunosorbent assay for measuring Human DDIMER in serum, plasma, and cell culture media.
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Cell Biolabs? Human D-dimer ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human D-dimer in plasma, serum or other biological fluid samples. The kit has a detection sensitivity limit of 150 pg/mL human D-dimer. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.
Description: Cell Biolabs? Human D-dimer ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human D-dimer in plasma, serum or other biological fluid samples. The kit has a detection sensitivity limit of 150 pg/mL human D-dimer. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Horse D-Dimer (D2D) in samples from Plasma with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Pig D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma.
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Diagnosis relies on magnetic resonance imaging, JCV detection in cerebrospinal fluid and, when necessary, brain histology. PML is often lethal. No specific, evidence-based treatment with clinically relevant efficacy is available. The therapeutic objective is to restore host immune responses to JCV, while avoiding immune-reconstitution inflammatory syndrome.
